E02M0209 Rat Macrophage MigRation Inhibitory Factor ELISA kit
The Rat Macrophage MigRation Inhibitory Factor ELISA kit can be used to identify samples from the rat species. Macrophage MigRation Inhibitory Factor can also be called Macrophage Migration Inhibitory Factor, GIF, GLIF, Glycosylation inhibiting factor, MIF protein, MMIF, Phenylpyruvate tautomerase, acrophage migration inhibitory factor (glycosylation-inhibiting factor), L-dopachrome isomerase, MIF.
Product Information | |
Cat. No. | E02M0209 |
Product Name | Rat Macrophage MigRation Inhibitory Factor ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MIF ELISA kit uses an anti-MIF antibody and an MIF-HRP conjugate in a competitive enzyme immunoassay method. MIF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MIF antibody binding site between MIF from samples and MIF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MIF. Since the number of sites is limited, as more sites are occupied by MIF from the sample, fewer sites are left to bind MIF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MIF and analogues was observed. | |
E02M0209 has been referenced in the below publications：
Therapeutic Effect of Simiaoyong＇an Ｒecipe Aqueous Extract and Its Influence on Peripheral Blood IL-1β，TNF-α and MIF Levels in Ｒats with Acute Gouty Arthritis.
中药熏洗治疗膝关节骨性关节炎的临床研究。
Study of circadian rhythm on anti--rheumatic in active ingredients of traditional Chinese medicine.
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