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BlueGene Biotech Rat Macrophage stimulating protein ELISA (E02M0006)

E02M0006 Rat Macrophage stimulating protein ELISA

The Rat Macrophage stimulating protein ELISAcan be used to identify samples from the rat species. Macrophage stimulating protein can also be called MST1, HGFL, NF15S2, Hepatocyte Growth Factor-Like Protein, Macrophage Stimulating 1, MSP.

Products

Specifications of Rat Macrophage stimulating protein ELISA

Product Information

Cat. No.

E02M0006

Product Name

Rat Macrophage stimulating protein ELISA

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MSP ELISA kit uses an anti-MSP antibody and an MSP-HRP conjugate in a competitive enzyme immunoassay method. MSP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MSP antibody binding site between MSP from samples and MSP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MSP. Since the number of sites is limited, as more sites are occupied by MSP from the sample, fewer sites are left to bind MSP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MSP concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Macrophage stimulating protein ELISA

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MSP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Macrophage stimulating protein ELISA

Summary of the Assay Procedure for Rat Macrophage stimulating protein ELISA

Citations of Rat Macrophage stimulating protein ELISA

E02M0006 has been referenced in the below publications:

Qingchang Wenzhong Decoction Attenuates DSS-Induced Colitis in Rats by Reducing Inflammation and Improving Intestinal Barrier Function via Upregulating the MSP/RON Signalling Pathway.


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