Rat Monocyte chemotactic protein 1 ELISA kit (E02M0246) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Rat Monocyte chemotactic protein 1 ELISA kit (E02M0246)

E02M0246 Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit

The Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit can be used to identify samples from the rat species. Monocyte chemotactic protein 1/monocyte chemotactic and activating factor can also be called C-C motif chemokine 2, CCL2, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je), Small inducible cytokine A2, Small inducible cytokine subfamily A (Cys Cys), member 2, Small inducible cytokine subfamily A Cys Cys member 2, Small-inducible cytokine A2, SMCCF, MCP 1.

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Specifications of Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit

Product Information
Cat. No.	E02M0246
Product Name	Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

MCP 1 ELISA kit uses an anti-MCP 1 antibody and an MCP 1-HRP conjugate in a competitive enzyme immunoassay method. MCP 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MCP 1 antibody binding site between MCP 1 from samples and MCP 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MCP 1. Since the number of sites is limited, as more sites are occupied by MCP 1 from the sample, fewer sites are left to bind MCP 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCP 1 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between MCP 1 and analogues was observed.

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Summary of the Assay Procedure for Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit

Citations of Rat Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA kit

E02M0246 has been referenced in the below publications：

Effect of losartan on the rats with myocardial fibrosis isoproterenol induced and the expression of inflammatory factor in isoproterenol rats.

Intrahepatic transplantation of adipose‑derived stem cells attenuates the progression of non‑alcoholic fatty liver disease in rats.