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BlueGene Biotech Rat Nuclear factor KB p50 ELISA kit (E02N0032)

E02N0032 Rat Nuclear factor KB p50 ELISA kit

The Rat Nuclear factor KB p50 ELISA kit can be used to identify samples from the rat species. Nuclear factor KB p50 can also be called NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit.

Products

Specifications of Rat Nuclear factor KB p50 ELISA kit

Product Information

Cat. No.

E02N0032

Product Name

Rat Nuclear factor KB p50 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NFkB p50 ELISA kit uses an anti-NFkB p50 antibody and an NFkB p50-HRP conjugate in a competitive enzyme immunoassay method. NFkB p50-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NFkB p50 antibody binding site between NFkB p50 from samples and NFkB p50-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NFkB p50. Since the number of sites is limited, as more sites are occupied by NFkB p50 from the sample, fewer sites are left to bind NFkB p50-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFkB p50 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Nuclear factor KB p50 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NFkB p50 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Nuclear factor KB p50 ELISA kit

Summary of the Assay Procedure for Rat Nuclear factor KB p50 ELISA kit

Citations of Rat Nuclear factor KB p50 ELISA kit

E02N0032 has been referenced in the below publications:

Comparative Analysis of NF-κB in the MyD88-Mediated Pathway After Implantation of Titanium Alloy and Stainless Steel and the Role of Regulatory T Cells.


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