Rat phosphorylated microtubule Associated protein tau ELISA Kit (E02P0005)

BlueGene Biotech Rat phosphorylated microtubule Associated protein tau ELISA Kit (E02P0005)

E02P0005 Rat phosphorylated microtubule Associated protein tau ELISA Kit

The Rat phosphorylated microtubule Associated protein tau ELISA Kit can be used to identify samples from the rat species. phosphorylated microtubule Associated protein tau can also be called p-Tau, Phospho-Tau (Ser409).

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Specifications of Rat phosphorylated microtubule Associated protein tau ELISA Kit

Product Information
Cat. No.	E02P0005
Product Name	Rat phosphorylated microtubule Associated protein tau ELISA Kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	250-5000 pg/mL
Sensitivity	1.0 pg/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	250 pg/mL	1 vial
STANDARD C (0.5mL)	500 pg/mL	1 vial
STANDARD D (0.5mL)	1000 pg/mL	1 vial
STANDARD E (0.5mL)	2500 pg/mL	1 vial
STANDARD F (0.5mL)	5000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

p-Tau ELISA kit uses an anti-Ip-Tau antibody and an Ip-Tau-HRP conjugate in a competitive enzyme immunoassay method. Ip-Tau-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Ip-Tau antibody binding site between Ip-Tau from samples and Ip-Tau-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Ip-Tau. Since the number of sites is limited, as more sites are occupied by Ip-Tau from the sample, fewer sites are left to bind Ip-Tau-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ip-Tau concentration in each sample is interpolated from this standard curve.

Quality Control on Rat phosphorylated microtubule Associated protein tau ELISA Kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between p-Tau and analogues was observed.

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Summary of the Assay Procedure for Rat phosphorylated microtubule Associated protein tau ELISA Kit

Citations of Rat phosphorylated microtubule Associated protein tau ELISA Kit

E02P0005 has been referenced in the below publications：

Alteration in amyloid β42, phosphorylated tau protein, interleukin 6, and acetylcholine during diabetes-accelerated memory dysfunction in diabetic rats: correlation of amyloid β42 with changes in glucose metabolism.

Alteration in amyloid β42,phosphorylated tau protein, interleukin 6,and acetylcholine during diabetes-accelerated memory dysfunction in diabetic rats: correlation of amyloid β42 with changes in glucose metabolism.