E02P0085 Rat Platelet Factor 4 ELISA kit
The Rat Platelet Factor 4 ELISA kit can be used to identify samples from the rat species. Platelet Factor 4 can also be called C-X-C motif chemokine 4, Chemokine (C X C motif) ligand 4, Chemokine (CXC motif) ligand 4, CXCL 4, CXCL4, Iroplact, Oncostatin A, Oncostatin-A, OncostatinA, PF-4, Platelet factor 4, SCYB 4, SCYB4, Small inducible cytokine subfamily member 4, PF4, PF 4.
Product Information | |
Cat. No. | E02P0085 |
Product Name | Rat Platelet Factor 4 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PF4 ELISA kit uses an anti-PF4 antibody and an PF4-HRP conjugate in a competitive enzyme immunoassay method. PF4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PF4 antibody binding site between PF4 from samples and PF4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PF4. Since the number of sites is limited, as more sites are occupied by PF4 from the sample, fewer sites are left to bind PF4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PF4 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PF4 and analogues was observed. | |
E02P0085 has been referenced in the below publications：
Effect of different compatibility of huayu-tongluo Chinese herbs on the kidney of diabetic nephropathy rats the expression of integrin alpha 3 beta 1 and the related proteins in wnt/beta-catenin pathway.
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