Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit (E02R0005)

BlueGene Biotech Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit (E02R0005)

E02R0005 Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

The Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit can be used to identify samples from the rat species. Receptor Activator of Nuclear Factor Kb Ligand can also be called CD254, TNFSF11, ODF, OPGL, TRANCE, HRANKL2, SOdf, Tumor Necrosis Factor(ligand)superfamily Member 11, TNF-related activation-induced cytokine, RANKL.

Products

Specifications of Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Product Information
Cat. No.	E02R0005
Product Name	Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/ml
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

RANKL ELISA kit uses an anti-RANKL antibody and an RANKL-HRP conjugate in a competitive enzyme immunoassay method. RANKL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-RANKL antibody binding site between RANKL from samples and RANKL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of RANKL. Since the number of sites is limited, as more sites are occupied by RANKL from the sample, fewer sites are left to bind RANKL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RANKL concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between RANKL and analogues was observed.

BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Citations of Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

E02R0005 has been referenced in the below publications：

Effects of combination of Tripterygium Glycoside with Ginsenoside on the proliferation and RANKL/OPG expression of rat fibroblast-like synoviocytes induced by Macrophage migration inhibitory factor.

The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats.