E02R0004 Rat Rheumatoid Factor ELISA kit
The Rat Rheumatoid Factor ELISA kit can be used to identify samples from the rat species. Rheumatoid Factor can also be called RF.
Specifications of Rat Rheumatoid Factor ELISA kit
Product Information | |
Cat. No. | E02R0004 |
Product Name | Rat Rheumatoid Factor ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 IU/ml |
Sensitivity | 1.0 IU/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 IU/mL | 1 vial |
STANDARD B (0.5mL) | 50 IU/mL | 1 vial |
STANDARD C (0.5mL) | 10 IU/mL | 1 vial |
STANDARD D (0.5mL) | 25 IU/mL | 1 vial |
STANDARD E (0.5mL) | 50 IU/mL | 1 vial |
STANDARD F (0.5mL) | 100 IU/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
RF ELISA kit uses an anti-IRF antibody and an IRF-HRP conjugate in a competitive enzyme immunoassay method. IRF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IRF antibody binding site between IRF from samples and IRF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IRF. Since the number of sites is limited, as more sites are occupied by IRF from the sample, fewer sites are left to bind IRF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IRF concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Rheumatoid Factor ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between RF and analogues was observed. | |
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Summary of the Assay Procedure for Rat Rheumatoid Factor ELISA kit
Citations of Rat Rheumatoid Factor ELISA kit
E02R0004 has been referenced in the below publications：
祖师麻肠溶制剂的药效学研究。
The effect of trichosanthis on the immune function of adjuvant-induced arthritis rats.
