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BlueGene Biotech Rat S100 Calcium Binding Protein A9 ELISA kit (E02S0039)

E02S0039 Rat S100 Calcium Binding Protein A9 ELISA kit

The Rat S100 Calcium Binding Protein A9 ELISA kit can be used to identify samples from the rat species. S100 Calcium Binding Protein A9 can also be called MRP14, CFAG, Leukocyte L1 complex heavy chain, CAGB, Calgranulin B, Calgranulin-B, CalgranulinB, CGLB, L1AG, Leukocyte L1 complex heavy chain, LIAG, MIF, Migration inhibitory factor related protein 14, NIF, P14, Calprotectin L1H subunit, CGLB, Cystic fibrosis antigen, MAC 387, MAC387, Migration inhibitory factor-related protein 14, MRP 14, MRP-14, MRP14, Protein S100-A9, S100 A9, S100 calcium binding protein A9, S100 calcium binding protein A9 calgranulin B, S100 calcium-binding protein A9, S100A9.

Products

Specifications of Rat S100 Calcium Binding Protein A9 ELISA kit

Product Information

Cat. No.

E02S0039

Product Name

Rat S100 Calcium Binding Protein A9 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/mL

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

S100A9 ELISA kit uses an anti-S100A9 antibody and an S100A9-HRP conjugate in a competitive enzyme immunoassay method. S100A9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-S100A9 antibody binding site between S100A9 from samples and S100A9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of S100A9. Since the number of sites is limited, as more sites are occupied by S100A9 from the sample, fewer sites are left to bind S100A9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A9 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat S100 Calcium Binding Protein A9 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between S100A9 and analogues was observed.


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Summary of the Assay Procedure for Rat S100 Calcium Binding Protein A9 ELISA kit

Summary of the Assay Procedure for Rat S100 Calcium Binding Protein A9 ELISA kit

Citations of Rat S100 Calcium Binding Protein A9 ELISA kit

E02S0039 has been referenced in the below publications:

Systemic Wound Healing Associated with local sub-Cutaneous Mechanical Stimulation.


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