Rat Secretoglobin, Family 1A, Member 1 ELISA Kit (E02U0019)

BlueGene Biotech Rat Secretoglobin, Family 1A, Member 1 ELISA Kit (E02U0019)

E02U0019 Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit

The Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit can be used to identify samples from the rat species. Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 can also be called SCGB1A1, CC10, CC16, CCPBP, CCSP, UGB, UP-1, UP1, secretoglobin family 1A member 1.

Products

Specifications of Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit

Product Information
Cat. No.	E02U0019
Product Name	Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

SCGB1A1/CC16 ELISA kit uses an anti-SCGB1A1/CC16 antibody and an SCGB1A1/CC16-HRP conjugate in a competitive enzyme immunoassay method. SCGB1A1/CC16-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SCGB1A1/CC16 antibody binding site between SCGB1A1/CC16 from samples and SCGB1A1/CC16-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SCGB1A1/CC16. Since the number of sites is limited, as more sites are occupied by SCGB1A1/CC16 from the sample, fewer sites are left to bind SCGB1A1/CC16-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCGB1A1/CC16 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between SCGB1A1/CC16 and analogues was observed.

BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit

Citations of Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit

E02U0019 has been referenced in the below publications：

Analysis of Components in Bronchoalveolar Lavage Fluid of Rats following silica exposure.

Study on levels of silicon and CC16 in serum of rats exposd to silica dust.