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BlueGene Biotech Rat Stromal cell Derived factor 1Alpha ELISA kit (E02S0006)

E02S0006 Rat Stromal cell Derived factor 1Alpha ELISA kit

The Rat Stromal cell Derived factor 1Alpha ELISA kit can be used to identify samples from the rat species. Stromal cell Derived factor 1Alpha can also be called SDF-1-alpha, SDF-1a, CXCL12a, SDF1A, SDF1Alpha.

Products

Specifications of Rat Stromal cell Derived factor 1Alpha ELISA kit

Product Information

Cat. No.

E02S0006

Product Name

Rat Stromal cell Derived factor 1Alpha ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

1.0 ng/ml

1 vial

STANDARD C (0.5mL)

2.5 ng/ml

1 vial

STANDARD D (0.5mL)

5.0 ng/ml

1 vial

STANDARD E (0.5mL)

10 ng/ml

1 vial

STANDARD F (0.5mL)

25 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SDF1Alpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SDF1Alpha. Standards or samples are then added to the microtiter plate wells and SDF1Alpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SDF1Alpha present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SDF1Alpha are added to each well to “sandwich” the SDF1Alpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SDF1Alpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF1Alpha concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Stromal cell Derived factor 1Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SDF1Alpha and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Stromal cell Derived factor 1Alpha ELISA kit

Summary of the Assay Procedure for Rat Stromal cell Derived factor 1Alpha ELISA kit

Citations of Rat Stromal cell Derived factor 1Alpha ELISA kit

E02S0006 has been referenced in the below publications:

The application of collagen-binding SDF and functional collagen scaffoldsfor spinal cord injury repair and myocardial regeneration.

Nano-Silicate-Reinforced and SDF-1α-Loaded Gelatin-Methacryloyl Hydrogel for Bone Tissue Engineering.

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