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BlueGene Biotech Rat Tartrate Resistant Acid Phosphatase ELISA kit (E02T0537)

E02T0537 Rat Tartrate Resistant Acid Phosphatase ELISA kit

The Rat Tartrate Resistant Acid Phosphatase ELISA kit can be used to identify samples from the rat species. Tartrate Resistant Acid Phosphatase can also be called ACP5, HPAP, SPENCDI, TRAP, TRACP5a, TRACP5b, TrATPase, acid phosphatase 5, tartrate resistant, TRAcP, TRAP.

Products

Specifications of Rat Tartrate Resistant Acid Phosphatase ELISA kit

Product Information

Cat. No.

E02T0537

Product Name

Rat Tartrate Resistant Acid Phosphatase ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TRAP ELISA kit uses an anti-TRAP antibody and an TRAP-HRP conjugate in a competitive enzyme immunoassay method. TRAP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TRAP antibody binding site between TRAP from samples and TRAP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TRAP. Since the number of sites is limited, as more sites are occupied by TRAP from the sample, fewer sites are left to bind TRAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TRAP concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Tartrate Resistant Acid Phosphatase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TRAP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Tartrate Resistant Acid Phosphatase ELISA kit

Summary of the Assay Procedure for Rat Tartrate Resistant Acid Phosphatase ELISA kit

Citations of Rat Tartrate Resistant Acid Phosphatase ELISA kit

E02T0537 has been referenced in the below publications:

Experimental Study of Herba Epimedii and Fructus Ligustri Lucid on the Glucocorticoid-induced Osteoporosis Rats based on regulation of TGF-beta 1/Smads signaling pathway.

麻黄、五味子配伍地塞米松对肺纤维化大鼠代谢差异性研究。

Combined Extracts of Herba Epimedii and Fructus Ligustri Lucidi Rebalance Bone Remodeling in Ovariectomized Rats.

Evaluation of the effect of CaD on the bone structure and bone metabolic changes in senile osteoporosis rats based on MLP-ANN methods.

Effects of the combined Herba Epimedii and Fructus Ligustri Lucidi on bone turnover and TGF-β1/Smads pathway in GIOP rats.


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