E02T0508 Rat Terminal complement complex C5b-9 ELISA kit
The Rat Terminal complement complex C5b-9 ELISA kit can be used to identify samples from the Rat species. Terminal complement complex C5b-9 can also be called MAC C5b-9, TCC C5b-9.
Specifications of Rat Terminal complement complex C5b-9 ELISA kit
Product Information | |
Cat. No. | E02T0508 |
Product Name | Rat Terminal complement complex C5b-9 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TCC C5b-9 ELISA kit uses an anti-TCC C5b-9 antibody and an TCC C5b-9-HRP conjugate in a competitive enzyme immunoassay method. TCC C5b-9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TCC C5b-9 antibody binding site between TCC C5b-9 from samples and TCC C5b-9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TCC C5b-9. Since the number of sites is limited, as more sites are occupied by TCC C5b-9 from the sample, fewer sites are left to bind TCC C5b-9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TCC C5b-9 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Terminal complement complex C5b-9 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TCC C5b-9 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Terminal complement complex C5b-9 ELISA kit
Citations of Rat Terminal complement complex C5b-9 ELISA kit
E02T0508 has been referenced in the below publications：
Effects of Schiff base aromatic amino acid derivatives on antioxidant and immune system disturbances in a rat model of aflatoxin B1 induced experimental mycotoxicosis.
