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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Terminal complement complex C5b-9 ELISA kit (E02T0508)

E02T0508 Rat Terminal complement complex C5b-9 ELISA kit

The Rat Terminal complement complex C5b-9 ELISA kit can be used to identify samples from the Rat species. Terminal complement complex C5b-9 can also be called MAC C5b-9, TCC C5b-9.

Products

Specifications of Rat Terminal complement complex C5b-9 ELISA kit

Product Information

Cat. No.

E02T0508

Product Name

Rat Terminal complement complex C5b-9 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TCC C5b-9 ELISA kit uses an anti-TCC C5b-9 antibody and an TCC C5b-9-HRP conjugate in a competitive enzyme immunoassay method. TCC C5b-9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TCC C5b-9 antibody binding site between TCC C5b-9 from samples and TCC C5b-9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TCC C5b-9. Since the number of sites is limited, as more sites are occupied by TCC C5b-9 from the sample, fewer sites are left to bind TCC C5b-9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TCC C5b-9 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Terminal complement complex C5b-9 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TCC C5b-9 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Terminal complement complex C5b-9 ELISA kit

Summary of the Assay Procedure for Rat Terminal complement complex C5b-9 ELISA kit

Citations of Rat Terminal complement complex C5b-9 ELISA kit

E02T0508 has been referenced in the below publications:

Effects of Schiff base aromatic amino acid derivatives on antioxidant and immune system disturbances in a rat model of aflatoxin B1 induced experimental mycotoxicosis.


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