E02T0317 Rat Thromboglobulin, β ELISA kit
The Rat Thromboglobulin, β ELISA kit can be used to identify samples from the rat species. Thromboglobulin, β can also be called beta-thromboglobulin, β-Thromboglobulin, bTG.
Specifications of Rat Thromboglobulin, β ELISA kit
Product Information | |
Cat. No. | E02T0317 |
Product Name | Rat Thromboglobulin, β ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
bTG ELISA kit uses an anti-bTG antibody and an bTG-HRP conjugate in a competitive enzyme immunoassay method. bTG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-bTG antibody binding site between bTG from samples and bTG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of bTG. Since the number of sites is limited, as more sites are occupied by bTG from the sample, fewer sites are left to bind bTG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The bTG concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Thromboglobulin, β ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between bTG and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Thromboglobulin, β ELISA kit
Citations of Rat Thromboglobulin, β ELISA kit
E02T0317 has been referenced in the below publications：
Effect of different compatibility of huayu-tongluo Chinese herbs on the kidney of diabetic nephropathy rats the expression of integrin alpha 3 beta 1 and the related proteins in wnt/beta-catenin pathway.
