E02T0008 Rat Tumor Necrosis Factor Alpha ELISA kit
The Rat Tumor Necrosis Factor Alpha ELISA kit can be used to identify samples from the rat species. Tumor Necrosis Factor Alpha can also be called DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2.
Product Information | |
Cat. No. | E02T0008 |
Product Name | Rat Tumor Necrosis Factor Alpha ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TNF-α ELISA kit uses an anti-TNF-α antibody and an TNF-α-HRP conjugate in a competitive enzyme immunoassay method. TNF-α-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TNF-α antibody binding site between TNF-α from samples and TNF-α-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TNF-α. Since the number of sites is limited, as more sites are occupied by TNF-α from the sample, fewer sites are left to bind TNF-α-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNF-α concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TNF-α and analogues was observed. | |
E02T0008 has been referenced in the below publications：
Protective effects of hydrogen-rich saline on monocrotaline-induced pulmonary hypertension in a rat model.
Therapeutic Effect of TSG-6 Engineered iPSC-Derived MSCs on Experimental Periodontitis in Rats: A Pilot Study.
Oxymatrine Improves Intestinal Epithelial Barrier Function Involving NF-kB-Mediated Signaling Pathway in CCl4-Induced Cirrhotic Rats.
Effects of chronic noise on glucose metabolism and gut microbiota-host inflammatory homeostasis in rats.
The Warburg effect in human pancreatic cancer cells triggers cachexia in athymic mice carrying the cancer cells.
Atrial Fibrillation Promotion in a Rat Model of Rheumatoid Arthritis.
The Mechanisms Underlying the Antidepressant Effect of Acupuncture via Regulating the Immune/Inflammatory Response Mediated by TLR4 Signaling Pathway/NLRP3 Inflammasome in Rats Exposed to Chronic Restraint Stress.
The research of the role of the p38MAPK in the acute lung injury induced by PO in rats.
Progesterone protects neurons against impairment induced by Aβ activated astrocytes.
Rat Interleukin 6 ELISA kit; Rat Interleukin 10 ELISA kit; Rat Connective Tissue Growth Factor ELISA kit; Rat Cyclooxygenase 2 ELISA kit; Rat Immunoglobulin G ELISA kit; Rat Prostaglandin E2 ELISA kit; Rat Tumor Necrosis Factor Alpha ELISA kit; Rat Transforming Growth Factor β ELISA kit; Rat Rheumatoid Factor ELISA kit.
THE STUDY OF FASUDIL HYDROCHLORIDE PREVENTED ACUTE KIDNEY INJURY RAT CAUSED BY LIPOPOLYSACCHARIDE.
Effects of Qingchang Wenzhong Decoction on intestinal mucosa barrier in colitis rats induced by TNBS.
A Modified Passive Smoking and Endo-tracheal Instillation of LPS Method for Establishment of COPD Models in Rats.
Untargeted Metabolomic Analysis of the Effects and Mechanism of Nuciferine Treatment on Rats With Nonalcoholic Fatty Liver Disease.
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