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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Chemokine C C motif ligand 8 ELISA kit (E14C0619)

E14C0619 Sheep Chemokine C C motif ligand 8 ELISA kit

Sheep Chemokine C C motif ligand 8 ELISA kit is suitable for the detection of samples from sheep species. Chemokine C C motif ligand 8 can also be called CCL8, HC14, MCP-2, MCP2, SCYA10, SCYA8, C-C motif chemokine ligand 8, CCL 8.

Products

Specifications of Sheep Chemokine C C motif ligand 8 ELISA kit

Product Information

Cat. No.

E14C0619

Product Name

Sheep Chemokine C C motif ligand 8 ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CCL 8 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CCL 8 antibody and an CCL 8-HRP conjugate. The assay sample and buffer are incubated together with CCL 8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL 8 concentration since CCL 8 from samples and CCL 8-HRP conjugate compete for the anti-CCL 8 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL 8 from the sample, fewer sites are left to bind CCL 8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL 8 concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Chemokine C C motif ligand 8 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CCL 8 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Chemokine C C motif ligand 8 ELISA kit

Summary of the Assay Procedure for Sheep Chemokine C C motif ligand 8 ELISA kit

Citations of Sheep Chemokine C C motif ligand 8 ELISA kit

E14C0619 has been referenced in the below publications:

Study on the Change of Early Serum and Intestinal Immune Factors in the Small Intestine of Kazak Sheep.

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