E14I0021 Sheep Immunoglobulin A ELISA kit
Sheep Immunoglobulin A ELISA kit is suitable for the detection of samples from sheep species. Immunoglobulin A can also be called Ig A, Immunoglobulin A, IgA.
Specifications of Sheep Immunoglobulin A ELISA kit
Product Information | |
Cat. No. | E14I0021 |
Product Name | Sheep Immunoglobulin A ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 mg/mL |
Sensitivity | 0.1 mg/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 mg/mL | 1 vial |
STANDARD C (0.5mL) | 5 mg/mL | 1 vial |
STANDARD D (0.5mL) | 10 mg/mL | 1 vial |
STANDARD E (0.5mL) | 25 mg/mL | 1 vial |
STANDARD F (0.5mL) | 50 mg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IgA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IgA. Standards or samples are then added to the microtiter plate wells and IgA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IgA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IgA are added to each well to “sandwich” the IgA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IgA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgA concentration in each sample is interpolated from this standard curve. |
Quality Control on Sheep Immunoglobulin A ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IgA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Sheep Immunoglobulin A ELISA kit
Citations of Sheep Immunoglobulin A ELISA kit
E14I0021 has been referenced in the below publications:
Study on the Change of Early Serum and Intestinal Immune Factors in the Small Intestine of Kazak Sheep.
Effects of α－Lipoic Acid on Growth Performance, Antioxidant and Immunity of Sheep under Heat Stress.
