E14I0038 Sheep Immunoglobulin M ELISA kit
Sheep Immunoglobulin M ELISA kit is suitable for the detection of samples from sheep species. Immunoglobulin M can also be called IGHM, Immunoglobulin Heavy Constant Mu, Ig mu chain C region, IgM.
Specifications of Sheep Immunoglobulin M ELISA kit
Product Information | |
Cat. No. | E14I0038 |
Product Name | Sheep Immunoglobulin M ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 mg/L |
Sensitivity | 1.0 mg/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/L | 1 vial |
STANDARD B (0.5mL) | 50 mg/L | 1 vial |
STANDARD C (0.5mL) | 100mg/L | 1 vial |
STANDARD D (0.5mL) | 250 mg/L | 1 vial |
STANDARD E (0.5mL) | 500 mg/L | 1 vial |
STANDARD F (0.5mL) | 1000 mg/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IgM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IgM antibody and an IgM-HRP conjugate. The assay sample and buffer are incubated together with IgM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgM concentration since IgM from samples and IgM-HRP conjugate compete for the anti-IgM antibody binding site. Since the number of sites is limited, as more sites are occupied by IgM from the sample, fewer sites are left to bind IgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgM concentration in each sample is interpolated from this standard curve. |
Quality Control on Sheep Immunoglobulin M ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IgM and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Sheep Immunoglobulin M ELISA kit
Citations of Sheep Immunoglobulin M ELISA kit
E14I0038 has been referenced in the below publications:
Study on the Changes of Serum IgE and IgM LeVels in Early Stage of Echinococcus granulosus Infection in Kazakh.
Study on the Change of Early Serum and Intestinal Immune Factors in the Small Intestine of Kazak Sheep.
