E14I0344 Sheep Interferon β ELISA kit
Sheep Interferon β ELISA kit is suitable for the detection of samples from sheep species. Interferon β can also be called IFNB1, IFN-B, IFB, IFF, IFNB, Interferon Beta 1 Fibroblast, IFN β, IFNβ.
Specifications of Sheep Interferon β ELISA kit
Product Information | |
Cat. No. | E14I0344 |
Product Name | Sheep Interferon β ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IFN β ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IFN β antibody and an IFN β-HRP conjugate. The assay sample and buffer are incubated together with IFN β-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFN β concentration since IFN β from samples and IFN β-HRP conjugate compete for the anti-IFN β antibody binding site. Since the number of sites is limited, as more sites are occupied by IFN β from the sample, fewer sites are left to bind IFN β-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFN β concentration in each sample is interpolated from this standard curve. |
Quality Control on Sheep Interferon β ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IFN β and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Sheep Interferon β ELISA kit
Citations of Sheep Interferon β ELISA kit
E14I0344 has been referenced in the below publications:
Protective effects of delayed intraventricular TLR7 agonist administration on cerebral white and gray matter following asphyxia in the preterm fetal sheep.
