En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Interleukin 2 ELISA kit (E14I0308)

E14I0308 Sheep Interleukin 2 ELISA kit

Sheep Interleukin 2 ELISA kit is suitable for the detection of samples from sheep species. Interleukin 2 can also be called Interleukin-2, IL-2, T-cell growth factor, Aldesleukin, Lymphokine, POIL2, T Cell Growth Factor, TCGF, IL2, IL 2.

Products

Specifications of Sheep Interleukin 2 ELISA kit

Product Information

Cat. No.

E14I0308

Product Name

Sheep Interleukin 2 ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL2 antibody and an IL2-HRP conjugate. The assay sample and buffer are incubated together with IL2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL2 concentration since IL2 from samples and IL2-HRP conjugate compete for the anti-IL2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL2 from the sample, fewer sites are left to bind IL2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL2 concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Interleukin 2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Interleukin 2 ELISA kit

Summary of the Assay Procedure for Sheep Interleukin 2 ELISA kit

Citations of Sheep Interleukin 2 ELISA kit

E14I0308 has been referenced in the below publications:

Study on the Change of Early Serum and Intestinal Immune Factors in the Small Intestine of Kazak Sheep.

The immunomodulatory on effect of recombinant ubiquitin ISG15 protein in infected Mycoplasma ovine Pneumoniae in argali hybrid sheep.

Assessment Model ofGeneral Disease Resistance With Principal Component Analysis in Sheeps.

The Comparison of Some Blood Immune Parameters in Sheep.

Identifcations of immune‑responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Sufolk sheep.

MHC-DRB1 exon 2 polymorphism and its association with mycoplasma ovipneumonia resistance or susceptibility genotypes in sheep.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.