E14I0007 Sheep Interleukin 4 ELISA kit
Sheep Interleukin 4 ELISA kit is suitable for the detection of samples from sheep species. Interleukin 4 can also be called IL4, BSF1, BCGF1 ,B Cell Stimulatory Factor 1, Lymphocyte stimulatory factor 1, B Cell Growth Factor, Binetrakin, Pitrakinra, IL 4, IL-4.
Product Information | |
Cat. No. | E14I0007 |
Product Name | Sheep Interleukin 4 ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL4 antibody and an IL4-HRP conjugate. The assay sample and buffer are incubated together with IL4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL4 concentration since IL4 from samples and IL4-HRP conjugate compete for the anti-IL4 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL4 from the sample, fewer sites are left to bind IL4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL4 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL4 and analogues was observed. | |
E11C0055 has been referenced in the below publications:
Study on the Change of Early Serum and Intestinal Immune Factors in the Small Intestine of Kazak Sheep.
The immunomodulatory on effect of recombinant ubiquitin ISG15 protein in infected Mycoplasma ovine Pneumoniae in argali hybrid sheep.
The immune regulation effect of bactericidal/permeability-increasing protein in argali hybrid sheep infected with Mycoplasma ovipneumoniae.
MHC-DRB1 exon 2 polymorphism and its association with mycoplasma ovipneumonia resistance or susceptibility genotypes in sheep.
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