E14I0024 Sheep Interleukin 5 ELISA kit
Sheep Interleukin 5 ELISA kit is suitable for the detection of samples from sheep species. Interleukin 5 can also be called B Cell Differentiation Factor I, IL5, IL 5, Colony stimulating factor, Colony Stimulating Factor Eosinophil, EDF, Eosinophil, Eosinophil Differentiation Factor, IL 5, Interleukin 5, B-cell differentiation factor I, IL-5, IL5_RAT, Interleukin-5, T Cell Replacing Factor, T-cell replacing factor, Interleukin5, TRF.
Product Information | |
Cat. No. | E14I0024 |
Product Name | Sheep Interleukin 5 ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL 5 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 5 antibody and an IL 5-HRP conjugate. The assay sample and buffer are incubated together with IL 5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 5 concentration since IL 5 from samples and IL 5-HRP conjugate compete for the anti-IL 5 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 5 from the sample, fewer sites are left to bind IL 5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 5 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL 5 and analogues was observed. | |
E14I0024 has been referenced in the below publications:
Assessment Model ofGeneral Disease Resistance With Principal Component Analysis in Sheeps.
The Comparison of Some Blood Immune Parameters in Sheep.
Identifcations of immune‑responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Sufolk sheep.
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