Name: Sheep Interleukin 6 ELISA kit (E14I0006)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Sheep Interleukin 6 ELISA kit (E14I0006)

E14I0006 Sheep Interleukin 6 ELISA kit

Sheep Interleukin 6 ELISA kit is suitable for the detection of samples from sheep species. Interleukin 6 can also be called Interleukin BSF 2, B cell differentiation factor, B cell stimulatory factor 2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor, Hepatocyte stimulating factor, HGF, HPGF, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL6 protein, Interferon beta 2, Interleukin 6 (interferon beta 2), Interleuk, IL 6.

Products

Specifications of Sheep Interleukin 6 ELISA kit

Product Information
Cat. No.	E14I0006
Product Name	Sheep Interleukin 6 ELISA kit
Species	Sheep
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/mL
Sensitivity	1.0 pg/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IL 6 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 6 antibody and an IL 6-HRP conjugate. The assay sample and buffer are incubated together with IL 6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 6 concentration since IL 6 from samples and IL 6-HRP conjugate compete for the anti-IL 6 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 6 from the sample, fewer sites are left to bind IL 6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 6 concentration in each sample is interpolated from this standard curve.

Quality Control on Sheep Interleukin 6 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	87-105%
Linearity	Diluent Ratio	Range %
	1:2	84-108
	1:4	81-107
	1:8	80-110
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IL 6 and analogues was observed.

BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Interleukin 6 ELISA kit

Citations of Sheep Interleukin 6 ELISA kit

E14I0006 has been referenced in the below publications:

Peripheral Injection of SB203580 Inhibits the Inflammatory-Dependent Synthesis of Proinflammatory Cytokines in the Hypothalamus.

The immunomodulatory on effect of recombinant ubiquitin ISG15 protein in infected Mycoplasma ovine Pneumoniae in argali hybrid sheep.

The immune regulation effect of bactericidal/permeability-increasing protein in argali hybrid sheep infected with Mycoplasma ovipneumoniae.

Assessment Model ofGeneral Disease Resistance With Principal Component Analysis in Sheeps.

Dopamine treatment during acute hypoxia is neuroprotective in the developing sheep brain.

Effects of α－Lipoic Acid on Growth Performance，Antioxidant and Immunity of Sheep under Heat Stress.

Identifcations of immune‑responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Sufolk sheep.

Twin Conception in Sheep Leads to Impaired Insulin Sensitivity and Sexually Dimorphic Adipose Tissue and Skeletal Muscle Phenotypes in Adulthood.