E14T0008 Sheep Tumor Necrosis Factor Alpha ELISA kit
Sheep Tumor Necrosis Factor Alpha ELISA kit is suitable for the detection of samples from sheep species. Tumor Necrosis Factor Alpha can also be called DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2. TNFAlpha. TNF Alpha, TNFα, TNF-α, TNF α.
Product Information | |
Cat. No. | E14T0008 |
Product Name | Sheep Tumor Necrosis Factor Alpha ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TNFAlpha ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TNFAlpha antibody and an TNFAlpha-HRP conjugate. The assay sample and buffer are incubated together with TNFAlpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNFAlpha concentration since TNFAlpha from samples and TNFAlpha-HRP conjugate compete for the anti-TNFAlpha antibody binding site. Since the number of sites is limited, as more sites are occupied by TNFAlpha from the sample, fewer sites are left to bind TNFAlpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNFAlpha concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TNFAlpha and analogues was observed. | |
E14T0008 has been referenced in the below publications:
Peripheral Injection of SB203580 Inhibits the Inflammatory-Dependent Synthesis of Proinflammatory Cytokines in the Hypothalamus.
Study on the Change of Early Serum and Intestinal Immune Factors in the Small Intestine of Kazak Sheep.
Lipopolysaccharide-Induced Preconditioning Attenuates Apoptosis and Differentially Regulates TLR4 and TLR7 Gene Expression after Ischemia in the Preterm Ovine Fetal Brain.
The Comparison of Some Blood Immune Parameters in Sheep.Effects of α－Lipoic Acid on Growth Performance，Antioxidant and Immunity of Sheep under Heat Stress.
Effects of α－Lipoic Acid on Growth Performance，Antioxidant and Immunity of Sheep under Heat Stress.
Twin Conception in Sheep Leads to Impaired Insulin Sensitivity and Sexually Dimorphic Adipose Tissue and Skeletal Muscle Phenotypes in Adulthood.
MHC-DRB1 exon 2 polymorphism and its association with mycoplasma ovipneumonia resistance or susceptibility genotypes in sheep.
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