E14I0345 Sheep Interferon γ ELISA kit
Sheep Interferon γ ELISA kit is suitable for the detection of samples from sheep species. Interferon γ can also be called IFNγ, IFG, IFI, IFN Gamma, IFNG.
Specifications of Sheep Interferon γ ELISA kit
Product Information | |
Cat. No. | E14I0345 |
Product Name | Sheep Interferon γ ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IFNγ ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IFNγ antibody and an IFNγ-HRP conjugate. The assay sample and buffer are incubated together with IFNγ-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNγ concentration since IFNγ from samples and IFNγ-HRP conjugate compete for the anti-IFNγ antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNγ from the sample, fewer sites are left to bind IFNγ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNγ concentration in each sample is interpolated from this standard curve. |
Quality Control on Sheep Interferon γ ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IFNγ and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Sheep Interferon γ ELISA kit
Citations of Sheep Interferon γ ELISA kit
E14I0345 has been referenced in the below publications:
Study on the Change of Early Serum and Intestinal Immune Factors in the Small Intestine of Kazak Sheep.
Assessment Model ofGeneral Disease Resistance With Principal Component Analysis in Sheeps.
The Comparison of Some Blood Immune Parameters in Sheep.
Effects of α－Lipoic Acid on Growth Performance，Antioxidant and Immunity of Sheep under Heat Stress.
Identifcations of immune‑responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Sufolk sheep.
MHC-DRB1 exon 2 polymorphism and its association with mycoplasma ovipneumonia resistance or susceptibility genotypes in sheep.
